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. 2000 May 30;97(12):6734–6738. doi: 10.1073/pnas.120081897

Figure 2.

Figure 2

Generation of PZ(−/−) mice. (A) Gene targeting. The targeting construct (Top) contains a neo cassette (neo) that replaces a 139-bp portion of exon 2 and induces a frameshift mutation. A herpes simplex virus–thymidine kinase cassette (tk) was added at the 3′ end of the mouse PZ DNA to permit negative selection. The predicted product of homologous recombination is shown (Bottom). The position of the ≈500-bp hybridization probe used to detect successful gene targeting is also depicted. (B) Southern blot analysis. Genomic DNA prepared from tail biopsies was analyzed by restriction digestion with XbaI and hybridization with the probe. Expected DNA fragment sizes are 9.0 kb for the wild-type allele and 3.2 kb for the disrupted allele. (C) Western blot analysis of mouse plasma with rabbit anti-human PZ polyclonal antibodies.