Figure 4.
In situ detection of T cell infiltration in carotid arteries of naive and preimmunized rats 3 days after balloon injury and viral infection. Viral solutions of Ad-βgal (1 × 108 pfu), Ad-FasL (1 × 108 pfu), or mixture of Ad-βgal and Ad-FasL (1 × 108 pfu each) were introduced into the balloon-injured carotid arteries of naive or immunized rats (n = 4 arteries for each group). Rats were immunized with an adenoviral vector lacking a transgene (1 × 109 pfu) 2 weeks prior to injury/infection. T cells were detected by using an anti-CD3 polyclonal antibody (arrows).