Figure 5.

Transgene expression in carotid arteries of naive and immunized rats 3 days after injury and viral infection. (A) β-Galactosidase and FasL expression in arteries infected with Ad-βgal (1 × 10⁸ pfu), Ad-FasL (1 × 10⁸ pfu), or a mixture of Ad-βgal (1 × 10⁸ pfu) and Ad-FasL (1 × 10⁸ pfu). β-Galactosidase was stained with 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (blue), while FasL expression was revealed by using an anti-FasL monoclonal antibody (Fast-Red detection system). The Ad-βgal encodes an Escherichia coli β-galactosidase containing a nuclear localization signal under the control of cytomegalovirus promoter. Arrows indicate β-galactosidase-expressing cells. (B) Mean transduction efficiencies of Ad-βgal (Left) or Ad-FasL (Right). Transduction efficiencies were calculated by determining the percentage of cells staining positive for the presence of transgenes (n = 4 for each group). The results are expressed as mean ± SEM.