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. 2000 Jun 6;97(12):6751–6756. doi: 10.1073/pnas.97.12.6751

Figure 5.

Figure 5

Immune response to OVA immunization in PDE4D+/+ and PDE4D−/− mice. Cellular composition of the BAL fluid from PDE4D+/+ and PDE4D−/− mice. Mice were immunized i.p. and i.n. with OVA as described in Fig. 2. Two days after the last immunization with OVA, the mice were bled and killed and the BAL was performed as detailed in Materials and Methods. BAL was performed with three aliquots of 0.8 ml serum-free medium. The total number of different types of leukocytes was determined from Hansel-Stain slide preparations of BAL fluid. The data are expressed as cells recovered ± SD/mouse of each cell type, based on differentials of 200 cells (n = 12 mice in each group). Mo, macrophages; Lym, lymphocytes; Eos, eosinophils; Neu, neutrophils.