Figure 6.

Immunolocalization of MAEBL to the merozoite apical complex by using scanning confocal laser microscopy. (B and D) Immunofluorescence with antibodies to the AMA-1-like domain M2 (A7) (B) and the EBP carboxyl cysteine-rich domain (YM C-CYS) (D) both localized MAEBL to the merozoite apical complex of P. yoelii yoelii YM. A and C are the nonconfocal transmitted images of B and D, respectively. The immunofluorescence pattern with each antibody was characteristic of a rhoptry protein as demonstrated by the bilobed structure present in numerous merozoites (arrows). (×3000.)