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. 2003 Sep;71(9):5254–5265. doi: 10.1128/IAI.71.9.5254-5265.2003

FIG. 6.

FIG. 6.

Western blot analysis of TAg recognized by intestinal IgA antibodies and MAbs. Fresh fecal samples were collected 21 days after infection of CBA/J mice with T. gondii cysts (strain 76K) (lanes 1 and 2), 21 days after injection of CBA/J mice with unpulsed SPDCs (lane 3), with TAg-pulsed SPDCs (lanes 4 and 5), with unpulsed MLNDCs (lane 6), or with TAg-pulsed MLNDCs (lanes 7 and 8). The following MAbs were used: 3G11 (anti-p22 [SAG2]) (lane a), 1E5 (anti-p30 [SAG1]) (lane b), 1F12 (anti-p43 [SAG3]) (lane c), 4A7 (anti-55- and 60-kDa [ROP2 to ROP4]) (lane d), 1F7 (anti-gp60 [MIC1]) (lane e), 2F3 (anti-p80 [MIC3]) (lane f), and 4A11 (anti-p100 [MIC2]) (lane g). The molecular masses (kilodaltons) of protein standards are given on the right.