FIG. 9.
Cellular proliferative response and cytokine production following i.v. immunization with 2.5 × 105 TAg-pulsed CBA/J SPDCs after challenge with 80 cysts of T. gondii 76K. At 28 days spleen and MLN cells were isolated and stimulated in vitro with TAg (10 μg/ml). Proliferation was assessed after 4 days. Values for IL-2 and IL-4 were measured at 24 h, for IFN-γ at 72 h and for IL-10 and IL-5 at 96 h of culture. Results are mean cytokine concentrations or counts per minute for proliferation assays on spleen and MLN cells from three mice per experimental group. Results from one of three similar experiments are shown and are expressed as the mean ± standard error of the mean. Symbols: *, P < 0.001; #, P < 0.001.