Cytokine secretion in response to fungal stimuli. TNF-α was measured in macrophage culture supernatants after 24-h incubation with C. albicans yeasts and hyphae and with A. fumigatus conidia and hyphae, each with an MOI of 10:1, in the presence of 10% heat-inactivated FBS. Results shown are means (± standard deviations) from triplicate measurements in one experiment, which was representative of three independent experiments. ND, not detectable (<15 pg/ml); *, P < 0.05 relative to MyD88+/+ control by Student's t test.