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. 2003 Sep;71(9):5280–5286. doi: 10.1128/IAI.71.9.5280-5286.2003

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FIG. 4. — Macrophage viability and function. (A) MyD88^+/+ and MyD88^−/− macrophage endocytosis was measured after a 1-h incubation with FITC-labeled dextran. Results are FITC mean fluorescence intensity (MFI) of lifted macrophages from triplicate measures in two independent experiments (mean ± standard deviation). Control represents FITC-dextran fluorescence of Na-azide-treated macrophages. (B) Macrophage viability was assessed at baseline and after a 1-h incubation with live C. albicans by flow-cytometric quantification of PI uptake. Results shown represent mean percentages of dead (PI-positive) macrophages in three independent experiments. More than 95% of the control (heat-killed) macrophages were PI positive (data not shown). (C) MyD88^+/+ and MyD88^−/− macrophages were allowed to phagocytose C. albicans yeasts for 1 h and then washed extensively and incubated in FITC-dextran for measurement of endocytosis. Results are FITC mean fluorescence intensity (MFI) of lifted macrophages from triplicate measures in two independent experiments (mean ± standard deviation). Control represents FITC-dextran fluorescence of Na-azide-treated macrophages.