FIG. 5.

SDS-PAGE and Western blot analyses of whole-cell lysates of S. uberis, showing detection of MtuA. Western blotting with antiserum raised against the CAL-n-FLAG-′MtuA fusion protein (A) and SDS-PAGE (B) of whole-cell lysates from S. uberis revealed a single reactive protein band at around 37 kDa (arrow) in strain 0140J (lane 2 in panel A) and no cross-reactive proteins in strain AJS001 (lane 3 in panel A) resulting from matched sample loadings (lanes 2 and 3 in panel B). Lane 1 contained molecular weight markers, and the mass of each marker protein (in kilodaltons) is indicated on the left.