Figure 2.

Expanded httex1p represses transcription. (Upper) SAOS-2 cells were transfected with vector alone (control) or plasmids encoding 25QP-GFP, 103QP-GFP, 25Q-myc, or 103Q-myc proteins and plasmids encoding WAF1-luciferase and p53. (Lower) SAOS-2 cells were transfected with vector alone (control) or plasmids encoding 20QP, 93QP, 25Q-myc, or 103-myc proteins and the MDR1-luciferase reporter plasmid. A p53-encoding plasmid was also transfected as indicated. Each experiment was done in triplicate. Luciferase units per milligram of protein were calculated and are demonstrated graphically as percent of vector control values.