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Deletion of CMP1 causes hypersensitivity to cationic and alkaline stresses. Serial 10-fold dilutions of the indicated strains were spotted on a YPD plate or YPD plates containing NaCl (1.8 M for URA3+ strains and 1.0 M for ura3 mutant strains), 0.3 M LiCl, 10 mM MnSO4, or 0.3 M CaCl2 and incubated at 30°C for 2 days. Alkaline pH sensitivity was tested on YPD plates buffered at pH 8.0.
