TABLE 2.
Serum IgG GAMP antibodies elicited in mice by three subcutaneous injections 2 weeks apart of conjugates in salinea
| Immunogen | Dose of PS (μg) | GM of IgG anti-GAMP | 25th-75th centiles |
|---|---|---|---|
| GAMPCNBr-AHBSA | 2.5 | 4.34 | 2.67-5.77 |
| GAMPCNBrAH-BSA | 2.5 | 8.50 | 7.11-11.3 |
| GAMPCDAP-AHBSA | 2.5 | 11.4 | 7.13-16.2 |
| GAMPCDAPAH-BSA | 2.5 | 5.16 | 3.31-9.43 |
| GAMPCNBrAH-BSA | 5.0 | 11.3 | 8.58-18.1 |
| GAMPCDAPAH-BSA | 5.0 | 6.68 | 4.30-11.8 |
| GAMP | 2.5 | 0.01 | 0.001-0.023 |
a
For recipients of 2.5 μg of conjugate, 11.4 versus 8.50, not significant; 11.4 versus 5.16 and 4.34, P < 0.01. The mice were injected three times subcutaneously with 0.1 (2.5 μg of PS) or 0.2 (5.0 μg of PS) ml of each conjugate every 2 weeks and exsanguinated 7 days after the third injection (n = 10/group). The sera were assayed for IgG anti-GAMP by ELISA, and the results are expressed as EU, using a hyperimmune serum assigned a value of 100 EU as a reference.