TABLE 3.
IgG anti-GAMP and bactericidal titers of individual sera from mice injected with GAMPCDAP-AHBSA and GAMP
Immunogen | Parametera | Value for mouse no.:
|
|||||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
GAMPCDAP-AHBSA | EU | 6.18 | 13.1 | 6.77 | 8.43 | 24.5 | 13.9 | 20.4 | 16.2 |
BC titer | 1,280 | 2,560 | 1,280 | 2,560 | 2,048 | 4,096 | 8,192 | 8,192 | |
Adsorbed | <20 | <20 | <20 | <20 | <20 | 20 | 40 | 40 | |
GAMP | EU | <0.01 | <0.01 | <0.01 | <0.01 | 0.01 | 0.01 | ||
BC titer | 64 | 8 | 64 | 8 | 64 | 512 |
Bactericidal (BC) activity is expressed as the reciprocal titer. Adsorption assays used GAMP (100 μg/ml of PBS) as the diluting buffer.