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. 2003 Sep;71(9):5324–5331. doi: 10.1128/IAI.71.9.5324-5331.2003

FIG. 1.

FIG. 1.

Freeze fracture of filipin-labeled host cell-free E. chaffeensis and A. phagocytophilum. Freeze-fractured replicas of filipin-labeled E. chaffeensis (EC) and A. phagocytophilum (AP) were examined by TEM. Intramembranous protuberances of filipin-cholesterol complexes in diameters of around 20 to 25 nm can be seen in the outer membranes (arrow 1) of filipin-treated EC and AP but are absent from the inner membrane (arrow 2) and the cytosol (arrow 3) of the bacteria. The bottom arrow indicates the direction of shadowing. Bar, 0.2 μm.