TABLE 1.
CD4+ IFN-γ+ and CD8+ IFN-γ+ T cells recruited to the site of intradermal challenge in C57BL/6 mice 6 months postvaccination
| Vaccination group | Total no. of cells/eara | Total no. of CD4+ IFN-γ+ cells (%)b | Total no. of CD8+ IFN-γ+ cells (%)b |
|---|---|---|---|
| Unvaccinated | 0.9 × 106 | 0.3 × 104 (0.3) | 0.3 × 104 (0.3) |
| ALM plus CpG ODNs | 2.0 × 106 | 4.7 × 104 (2.3) | 11 × 104 (5.5) |
| Antigen DNA | 1.2 × 106 | 4.3 × 104 (3.5) | 3.2 × 104 (2.6) |
| L. major and ALM plus CpG ODNs | 2.8 × 106 | 47 × 104 (16.7) | 18 × 104 (6.4) |
a
Dermal cells were extracted from mouse ears 48 h after intradermal injection of 106 metacyclic promastigotes plus 12.5 μg of soluble leishmanial antigen, and the number of cells per ear was calculated from cell preparations pooled from three to four mice (six to eight ears) for each group.
b
Double-positive cells as a percentage of total number of extracted cells, determined by fluorescence-activated cell sorter analysis following 24-h in vitro restimulation using L. major-infected BMDDC.