TABLE 2.
Humoral immune response induced by PorA liposomes and OMV
| Antigen | Whole-cell ELISA titera
|
Bactericidal assay
|
|||||
|---|---|---|---|---|---|---|---|
| IgG | IgG1 | IgG2a | IgG2b | IgG3 | % Responders | Titerb | |
| OMV | 3.40 | 3.16 | 3.21 | 3.52 | 2.87 | 62.5 | 2.38 ± 0.29 |
| PG liposomes | 3.34 | 3.19 | 3.15 | 3.49 | 2.77 | 50 | 2.25 ± 0.52 |
| Man liposomes | 3.26 | 2.98 | 3.15 | 3.30 | 2.84 | 87.5 | 2.30 ± 0.49 |
| PS liposomes | 3.40 | 3.01 | 3.20 | 3.33 | 2.78 | 100 | 1.99 ± 0.47 |
| TAP liposomes | 3.34 | 3.09 | 3.51 | 3.56 | 2.71 | 87.5 | 1.88 ± 0.54 |
a
The titer of each anti-PorA P1.7-2,4 IgG isotype was determined by whole-cell ELISA and is expressed as the mean log10 titer. The means were compared by the LSD test with a confidence level of 95%: LSD0.05 (IgG) = 0.23; LSD0.05 (IgG1) = 0.35; LSD0.05 (IgG2a) = 0.29; LSD0.05 (IgG2b) = 0.3; LSD0.05 (IgG3) = 0.35.
b
The bactericidal titer was determined as the reciprocal value of the serum dilution that effectuates killing of >90% of strain H44/76 7-24 and is expressed as the averaged mean log10 titer in the responders ± standard deviation.