FIG. 3.

Western blot of the affinity-purified serotype d-specific antigen. The serotype d-specific antigen was isolated as described in Materials and Methods. The affinity-purified serotype d fraction and the control fraction were subjected to SDS-12.5% PAGE, transferred to a nitrocellulose membrane, and treated with polyclonal rabbit antisera raised against A. actinomycetemcomitans serotypes a through e. Lane 1, control fraction detected with rabbit antiserum raised against serotype c. Lanes 2 to 6, affinity-purified serotype d-specific antigen detected with rabbit antisera raised against serotypes a through e, respectively.