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. 2003 Sep;71(9):4831–4841. doi: 10.1128/IAI.71.9.4831-4841.2003

FIG. 2.

FIG. 2.

Morphological features of C. neoformans var. grubii mating. The a mating type, serotype A strain 125.91 was mixed with the α mating type, serotype A strain 8-1. (A) Example of filaments observed after 10 days of growth on V8 (pH 5.0) at 25°C in the dark. Bar, 1 mm. (B) Example of filaments, basidia, and spores produced after 10 days of growth on V8 (pH 5.0) at 25°C in the dark. Bar, 25 μm. (C) DAPI staining of nuclei in filaments produced in a 125.91 (aA)-to-8-1 (αA) cross. Arrows designate paired nuclei in the dikaryons. Bar, 25 μm. (D) Differential contrast image of the filaments seen in panel C. (E) Calcofluor White staining of the septa of filaments produced in a 125.91 (aA)-to-8-1 (αA) cross. The arrowhead designates a fused clamp connection. Bar, 25 μm. (F) DIC image of the filament seen in panel E.