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. 2003 Sep;71(9):4831–4841. doi: 10.1128/IAI.71.9.4831-4841.2003

FIG. 3.

FIG. 3.

RFLP analysis of progeny from C. neoformans var. grubii crosses shows recombination. The presence or absence of the PvuI restriction site in the GPA1 gene and the BstXI restriction site in the PAK1 gene was examined by digestion of the PCR-amplified gene with the appropriate enzyme. An asterisk indicates the strain used to generate the serotype A congenic strains. (A) RFLP analysis of the parental strains 125.91 (aA) and 8-1 (αA) and progeny from a cross of the parental strains. Strain KNA14 was used to generate the serotype A congenic strains. (B) RFLP analysis of the parental strains KNA14 (aA) and H99 crg1 (αA) and progeny from a cross of the parental strains. KNB-5 was used to generate the serotype A congenic strains. (C) RFLP analysis of the parental strains KNA14 (aA) and H99 (αA), the 5th-backcross strain KN99-5a, and the 10th-backcross strains KN99a and KN99α.