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The crg1 mutation enhances pheromone response. The a and α strains were grown in confronting lines ∼1 mm apart on SLAD medium and incubated at 25°C in the dark for 72 h. Strains were examined microscopically for the presence of conjugation tubes. aD, JEC20; αD, JEC21; αA, H99; αA crg1, H99 crg1. Bar, 1 mm.
