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Full-length APP is not increased in apoptotic neurons. Cells were labeled and apoptosis was triggered as described in Fig. 2. Cells were lysed and total proteins were immunoprecipitated with the C7 antibody and electrophoresed on 10% gels in glycine. Notice that full-length APP (130 kDa) is equal in control neurons (lane 2) and apoptotic cells (lane 1).
