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Purification of APE97 from B. melitensis
| Purification step | Amt of protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 901.0 | 998.6 | 1.1 | 100 | 1.0 |
| Ammonium sulfate (40-70%) | 542.1 | 773.3 | 1.4 | 77.0 | 1.3 |
| High-performance Q Sepharose | 40.0 | 502.0 | 12.5 | 50.0 | 11.3 |
| Phenyl Sepharose | 4.2 | 411.2 | 98.0 | 41.1 | 89.0 |
| Superose 12HR | 1.8 | 286.8 | 159.0 | 29.0 | 144.5 |
