TABLE 2.
Response of CD4+-T-cell lines from RAP-1 and RAP-1 NT vaccinees to NT region peptides
| Antigen or peptide | Proliferation (SI) by T-cell lines from calvesa
|
|||||||
|---|---|---|---|---|---|---|---|---|
| RAP-1 vaccinees
|
RAP-1 NT vaccinees
|
|||||||
| 45 (27/23b) | 46 (16/14) | 51 (27/24) | 55 (8/24) | 47 (27/14) | 48 (16/22/26) | 52 (16/24) | 53 (23/11) | |
| RAP-1 | 22.5 | 52.3 | 275.8 | 69.3 | 77.3 | 9.3 | 169.3 | 69.2 |
| RAP-1 NT | 4.3 | 45.0 | 164.3 | 64.5 | 75.6 | 9.7 | 174.2 | 84.4 |
| N2 | 5.6 | 35.9 | 4.6 | 3.6 | 77.3 | 2.9 | 12.0 | 4.7 |
| P0 | 1.0 | 29.2 | 109.1 | 71.9 | 365.0 | 1.2 | 177.0 | 7.6 |
| 60-0 | 1.1 | 1.6 | 0.5 | 0.7 | 1.3 | 0.8 | 0.8 | 1.6 |
| P1 | 1.6 | 6.3 | 0.6 | 28.6 | 107.6 | 1.0 | 21.9 | 24.7 |
| P2 | 1.7 | 34.4 | 0.2 | 55.1 | 347.3 | 0.9 | 1.0 | 2.5 |
| P3 | 1.9 | 1.7 | 28.1 | 43.9 | 325.0 | 5.4 | 18.1 | 140.4 |
| P4 | 2.0 | 17.3 | 18.9 | 41.7 | 414.7 | 1.5 | 3.2 | 147.6 |
| P5 | 1.7 | 41.1 | 0.9 | 2.2 | 2.8 | 4.9 | 172.7 | 156.8 |
| P6 | 1.8 | 23.7 | 1.5 | 1.6 | 301.9 | 10.2 | 111.7 | 53.2 |
| P7 | 9.6 | 45.1 | 18.3 | 5.1 | 211.0 | 1.1 | 154.0 | 143.9 |
| P8 | 1.0 | 11.2 | 0.9 | 18.3 | 356.5 | 7.4 | 31.6 | 49.9 |
| P9 | 1.4 | 30.0 | 2.8 | 23.1 | 238.1 | 0.9 | 1.9 | 33.5 |
Cell lines were stimulated for 1 week with B. bovis CM and rested for 1 week before being tested. The results are reported for the optimal response to 1 or 10 μg of recombinant protein or peptide/ml. SIs were calculated by dividing the mean counts per minute of the response to RAP-1 or RAP-1 NT by the mean counts per minute of the response to MSP-5 or by dividing the mean counts per minute of the response to NT peptides by the mean counts per minute of the response to the control MSP2 peptide, P1. Significant responses (SI ≥ 3.0) are indicated in boldface type. These responses were also significant when individual counts per minute were compared by the one-tailed Student t test (P < 0.05).
DRB3 haplotypes for the indicated calves. Calf 48 was apparently a chimeric twin.