TABLE 4.

B. bovis RAP-1-specific IgG1 and IgG2 responses in calves following immunization

Immunogen and calf no.	Antibody titera
	B. bovis CM		RAP-1 NT		RAP-1 CT
	IgG1	IgG2	IgG1	IgG2	IgG1	IgG2
RAP-1
45	12,500	Negb	2,500	Neg	12,500	Neg
46	12,500	12,500	12,500	12,500	12,500	12,500
51	12,500	12,500	12,500	12,500	2,500	2,500
55	2,500	2,500	2,500	2,500	12,500	2,500
RAP-1 NT
47	12,500	Neg	2,500	Neg	NDc	ND
48	12,500	12,500	12,500	12,500	ND	ND
52	12,500	12,500	12,500	12,500	ND	ND
53	2,500	Neg	2,500	Neg	ND	ND

^aAntibody titer in sera collected after the fourth immunization is expressed as the reciprocal of the highest dilution that gave a positive signal on immunoblots against specific antigen.

^bNeg, titer of <100, the lowest serum dilution tested.

^cND, not determined.