TABLE 7.
RAP-1-specific proliferation by CD4+ T lymphocytes after B. bovis challenge
| Group and calf no.a | Proliferation (SI) againstb:
|
|||
|---|---|---|---|---|
| B. bovis CM | RAP-1 | RAP-1 NT | RAP-1 CT | |
| RAP-1 (II) | ||||
| 45 (27/23) | 39.3 | 59.9 | 15.1 | 58.3 |
| 46 (16/14) | 74.0 | 82.4 | 33.8 | 75.8 |
| 51 (27/24) | 16.3 | 26.2 | 25.7 | 23.2 |
| 55 (8/24) | 6.5 | 16.0 | 14.9 | 4.2 |
| RAP-1 NT (III) | ||||
| 47 (27/14) | 135.9 | 10.8 | 4.5 | 13.0 |
| 52 (16/24) | 19.1 | 76.8 | 76.7 | 5.4 |
| 53 (23/11) | 205.0 | 124.7 | 107.8 | 95.8 |
| PBS(I) | ||||
| 49 (11/27) | 431.2 | 15.7 | 5.3 | 13.9 |
| 54 (27/7) | 114.7 | 159.8 | 0.8 | 209.0 |
| 60 (16/22) | 0.8 | 2.4 | 0.8 | 2.0 |
| 61 (11/28) | 86.2 | 133.5 | 29.4 | 7.2 |
DRB3 haplotypes for the indicated calves are in parentheses.
CD4+-T-cell lines were set up 6 weeks after challenge of immunized cattle and 5 months after challenge of control calves. T cells were cultured for 1 week with B. bovis CM (calf 60) or for 1 week with B. bovis CM and 1 week without antigen (other calves). Lymphocytes were tested against the indicated antigens, and SIs are reported for the optimal response to 1 or 10 μg of antigen /ml. SIs were calculated as the mean counts per minute to B. bovis CM divided by the mean counts per minute to URBC or as the mean counts per minute to recombinant RAP-1, RAP-1 NT, or RAP-1 CT divided by the mean counts per minute to MSP-5. Significant responses (SI ≥ 3.0) are indicated in boldface type. These responses were also significant when individual counts per minute were compared by the one-tailed Student t test (P < 0.05).