TABLE 8.
Responses of CD4+ T cell lines to NT and CT region peptides after challenge
| Peptide | Proliferation (SI) by T-cell lines from calvesa
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RAP-1 vaccinees
|
RAP-1 NT vaccinees
|
Controls
|
||||||||
| 45 | 46 | 51 | 55 | 47 | 52 | 53 | 49 | 54 | 61 | |
| NT peptides | ||||||||||
| P0 | 1.4 | 53.3 | 35.7 | 19.4 | 5.7 | 33.3 | 95.6 | 4.1 | 0.8 | 1.1 |
| 60-0 | 1.4 | 1.3 | 2.2 | 2.8 | 1.7 | 1.8 | 1.3 | 0.7 | 2.2 | 1.0 |
| P1 | 1.4 | 2.5c | 4.6b | 10.9 | 1.8c | 1.1c | 2.7c | 1.0 | 2.1 | 1.0 |
| P2 | 2.1 | 27.6 | 2.3 | 15.0 | 2.1c | 6.0b | 3.5 | 1.0 | 6.4 | 1.5 |
| P3 | 4.4b | 0.4 | 7.6 | 11.7 | 1.2c | 7.9 | 79.8 | 29.5 | 1.2 | 11.0 |
| P4 | 3.5b | 2.1c | 7.9 | 1.9c | 1.0c | 2.0c | 86.0 | 5.1 | 2.6 | 0.6 |
| P5 | 1.6 | 18.3 | 2.9 | 1.1 | 1.2 | 25.0 | 75.3 | 1.1 | 2.4 | 0.4 |
| P6 | 1.8 | 1.4c | 2.0 | 1.7 | 1.0c | 2.2c | 2.2c | 2.4 | 8.2 | 0.4 |
| P7 | 64.2 | 3.6 | 8.0 | 4.0 | 1.5c | 14.2 | 119.9 | 3.2 | 1.9 | 1.6 |
| P8 | 2.1 | 0.9c | 1.0 | 1.8c | 1.1c | 1.3c | 0.9c | 2.3 | 1.3 | 0.5 |
| P9 | 2.7 | 29.9 | 2.6 | 6.0 | 1.6c | 2.6 | 4.3 | 0.8 | 2.1 | 1.0 |
| CT peptides | ||||||||||
| CT1 | 1.7 | 3.4 | 1.5 | 2.6 | 1.11 | 2.7 | 1.6 | 1.9 | 0.8 | 1.6 |
| CT2 | 1.9 | 1.0 | 1.6 | 1.4 | 1.4 | 0.6 | 1.0 | 3.0 | 1.1 | 0.8 |
| CT3 | 74.5 | 1.5 | 1.9 | 3.4b | 1.5 | 3.2b | 21.7b | 2.2 | 1.0 | 0.9 |
| CT4 | 21.3 | 1.3 | 2.6 | 2.0 | 1.9 | 10.0b | 1.2 | 1.9 | 46.4 | 2.0 |
| CT5 | 76.7 | 1.4 | 1.6 | 2.9 | 1.7 | 4.3b | 118.3b | 13.0 | 0.8 | 14.2 |
| CT6 | 72.6 | 1.2 | 1.8 | 2.7 | 0.8 | 0.9 | 110.8b | 4.3 | 43.8 | 8.5 |
| CT7 | 79.4 | 31.4 | 1.3 | 3.3b | 1.1 | 1.8 | 117.9b | 4.4 | 22.3 | 2.1 |
| CT8 | 2.1 | 28.1 | 2.3 | 3.3b | 1.2 | 2.8 | 14.7b | 1.9 | 12.9 | 0.9 |
| CT9 | 77.6 | 34.1 | 33.3 | 4.2b | 3.2b | 2.1 | 25.9b | 38.1 | 84.9 | 1.2 |
| CT10 | 28.8b | 131.6 | 28.9 | 6.8 | 26.2b | 2.0 | 1.0 | 1.4 | 2.0 | 1.0 |
| CT11 | 2.0 | 1.8 | 1.8 | 3.6b | 1.4 | 1.4 | 0.9 | 1.9 | 5.8 | 1.3 |
| CT12 | 1.8 | 1.1 | 3.1 | 2.6 | 1.2 | 1.1 | 1.6 | 1.6 | 0.9 | 1.4 |
| CT13 | 80.1 | 1.0 | 1.8 | 3.0 | 0.9 | 2.9 | 0.9 | 1.9 | 0.7 | 1.8 |
CD4+-T-cell lines were set up 6 weeks after challenge of immunized cattle and 5 months after challenge of control cattle. T cells were cultured for 1 week with B. bovis CM and 1 week without antigen. Lymphocytes were tested against the indicated peptides, and SIs are reported for the optimal response to 1 or 10 μg of peptide /ml. SIs were calculated as the mean counts per minute to peptide divided by the mean counts per minute to control the MSP2 peptide, P1. Significant responses (SI ≥ 3.0) are indicated in boldface type. These responses were also significant when individual counts per minute were compared by the one-tailed Student t test (P < 0.05).
Responses were negative before challenge.
Responses were positive before challenge.