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Expression of TLR5 protein in murine osteoblasts. Cells were unexposed or exposed to purified flagellin (1, 10, and 100 ng/ml) for 12 h. Whole-cell lysates were prepared and analyzed by Western blotting for the presence of TLR5. Below, densitometric analysis of this representative experiment is shown as arbitrary densitometric units corrected for background intensity for each lane. These studies were performed three times with similar results.
