FIG. 1.

Effect of PmxB on activity of B. cepacia LPS to stimulate murine peritoneal macrophages for production of TNF-α and IL-6. B. cepacia LPS (A and C) or reference S. enterica serovar Abortusequi LPS (B and D) was incubated with PmxB (•) in PFDW at a ratio of 1:500 (LPS-PmxB, by weight) or without PmxB (○) at room temperature for 30 min. The specimens were added to the culture of peritoneal macrophages of C3H/HeN mice by diluting them appropriately with the culture medium to the final LPS concentrations indicated. The cells were cultured, and the culture supernatants were obtained at 4 and 48 h for determination of TNF-α (A and B) and IL-6 (C and D), respectively. The data are the means ± standard errors of the mean of triplicate samples. A representative result obtained from three independent experiments is shown.