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. 2003 Sep;71(9):4953–4960. doi: 10.1128/IAI.71.9.4953-4960.2003

FIG. 2.

FIG. 2.

Northern blot analysis. (A) Total RNA was isolated from mating cultures of MATa (B-4476FO5) and MATα (JEC33) strains on V-8 agar or YEPD plates for indicated times. The probes used in each hybridization are as specified. (B) RNA was isolated from cells of a MATa strain (B-4476FO5), which were transferred from YEPD liquid (0 h) onto V-8 agar (V8 6 h) or YEPD plate (YEPD 6 h). (C) RNA was isolated from cells of the wild type (wt, B-4476FO5), ste12a deletion mutant (Δste12a, TYCC384), or cpra deletion mutant (Δcpra, TYCC419), which were transferred from YEPD liquid onto V-8 agar plates (P) or to V-8 liquid medium (L). (D) RNA was isolated from cells from mating cultures containing either the wild type (WT X WT, JEC33 × B-4476FO5), ste12a deletion mutant (WT X Δste12a, JEC33 × TYCC384) or cpra deletion mutant (WT X Δcpra, JEC33 × TYCC419).