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Cellular immune response kinetics to M. avium subsp. paratuberculosis strain 19698 WCS. Mean (± SEM) DNA synthesis (A), IFN-γ (B), and NO (C) responses by PBMC from M. avium subsp. paratuberculosis-infected calves (closed squares) (n = 3) are depicted. DNA synthesis was measured by [3H]thymidine uptake, IFN-γ was measured by ELISA, and nitrite (as an indication of NO synthesis) was measured by Griess reaction. *, Differs (P < 0.05) from response at day 0 (i.e., prechallenge response.
