TABLE 2.
Percent expression of CD45RO and CD26 on CD4+ cells from M. avium subsp. paratuberculosis- infected cattle upon antigen stimulation
| Subseta | % Expression
|
|||
|---|---|---|---|---|
| Freshly isolated | Medium | PPDa | WCS | |
| CD45RO− | 42.3 (2.85) | 52.3 (3.18) | 4.0 (0)*** | 3.0 (0.58)*** |
| CD45RO+ | 56.7 (2.33) | 45.3 (2.60) | 95.3 (0.67)*** | 97.0 (1.00)*** |
| CD26− | 70.3 (1.86) | 89.7 (2.73) | 19.5 (6.5)** | 30.3 (11.85)* |
| CD26+ | 29.3 (2.19) | 7.3 (3.38) | 80.5 (6.50)** | 69.3 (12.17)* |
a
At 286 days postchallenge, PBMC were cultured with either medium alone, 4 μg of PPDa/ml, or 20 μg of strain K10 WCS/ml for 7 days, harvested, and analyzed by flow cytometry. Data represent CD26 or CD45RO expression [mean percent positive or negative (± SEM)] by CD4+ cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; differs from response to media alone and expression by freshly isolated PBMC, same subset (i.e., horizontal comparisons).