TABLE 2.

Oligonucleotides used in this study

Oligonucleotidea	Sequence (5′-3′)	Usage
E54.1	AATGGTATGTTCAATGACC	Expression
E54.2	AGAAGCACGCGAACAAGCA	Expression
E54.3	TCTGCTTGTTCGCGTGCTT	Expression
E54.4	CACCATTCACACTTCCAGA	Expression
E54.5	CATGCTGACAGCAAAGTCA	Expression
Eh54.1	GTCGGAGGAATGAAGAGT	Disruption
Eh54.3	GTTGACGAGCTTGTTCTG	Disruption
sagAup	CGGAATTCGAAATGCTAGACTTAACGC	Complementation
sagAdown	CGGAATTCGCAAGCAGGTATCGACATG	Complementation

^aE54.1 and E54.5 were used to express the full-length SagA (F). E54.1 and E54.3 were used to express the N-terminal domain of SagA (N). E54.1 and E54.4 were used to express the N-terminal half of SagA (containing the N-terminal and middle domains [NM]). E54.2 and E54.4 were used to express the middle (repeat) domain of SagA (M). E54.2 and E54.5 were used to express the C-terminal half of SagA (containing middle and C-terminal domains [MC]). Letter codes correspond to lane designations in Fig. 5.