FIG. 3.

Expression of yF2 (Pichia codon-optimized protein) in P. pastoris. (A) yF2 was detectable in reduced (lane 1) and nonreduced (lane 2) culture supernatants upon Coomassie blue staining. (B) Coomassie blue staining of yF2 purified over Ni-NTA, showing the presence of the monomeric form of the protein (lane 1), which had a reduced mobility following reduction (lane 2). (C) Nonreduced yF2 was recognized to an equal extent by a conformational MAb (lane 1) and polyclonal anti-yF2 antiserum (lane 3). Reduced yF2 was recognized by the polyclonal antibodies (lane 4) but was not recognized by the MAb (lane 2).