TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| M. catarrhalis | ||
| O35E | Wild-type isolate | 22 |
| O35E.1 | uspA1 isogenic mutant of strain O35E | 1 |
| O35E.TN2 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN19 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN34 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN107 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN114 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN115 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN184 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN246 | Adherence negative hag transposon mutant of strain O35E | This study |
| O35E.TN532 | Adherence negative hag transposon mutant of strain O35E | This study |
| E. coli EPI300 | Cloning strain | Epicentre |
| Plasmids | ||
| pCC1 | Cloning vector | Epicentre |
| pUC4K | Source of the Kanr cassette | AmershamBiosciences |
| pMHO35ETN2 | pCC1 containing the transposon and flanking M. catarrhalis DNA of strain O35E.TN2 | This study |
| pMHO35ETN114 | pCC1 containing the transposon and flanking M. catarrhalis DNA of strain O35E.TN114 | This study |
| pMHO35ETN532 | pCC1 containing the transposon and flanking M. catarrhalis DNA of strain O35E.TN532 | This study |