TABLE 2.
Adherence of M. catarrhalis strains to human cell monolayers in vitro
| M. catarrhalis strain | Description | UspA1 expressiona | Hag expressiona | % Adherence (±SD) to:
|
||
|---|---|---|---|---|---|---|
| A549 cells | Chang cells | HMEE cells | ||||
| O35E | Wild type | + | + | 33.7 ± 1.6 | 22.6 ± 5.7 | 17.6 ± 6.8 |
| O35E.1 | uspA1 mutant | − | + | 28.1 ± 6.1 | 4.2 ± 2.6b | 13.1 ± 7.1 |
| O35E.TN2 | TN mutant | + | − | 1.8 ± 1.1b | 23.6 ± 2.1 | 0.4 ± 0.2b |
| O35E.TN19 | TN mutant | + | − | 2.0 ± 1.1b | NDc | ND |
| O35E.TN34 | TN mutant | + | − | 2.2 ± 0.3b | ND | ND |
| O35E.TN107 | TN mutant | + | − | 2.3 ± 2.2b | ND | ND |
| O35E.TN114 | TN mutant | + | − | 1.9 ± 1.3b | ND | ND |
| O35E.TN115 | TN mutant | + | − | 3.9 ± 2.8b | ND | ND |
| O35E.TN184 | TN mutant | + | − | 2.2 ± 1.5b | ND | ND |
| O35E.TN246 | TN mutant | + | − | 2.7 ± 2.0b | ND | ND |
| O35E.TN532 | TN mutant | + | − | 3.7 ± 2.2b | ND | ND |
a
As determined by Western blot analysis.
b
The difference in the P value compared to the value for wild-type strain O35E was found to be statistically significant using a Student t test.
c
ND, not determined.