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. 2003 Sep;71(9):5407–5411. doi: 10.1128/IAI.71.9.5407-5411.2003

FIG. 2.

FIG. 2.

Specific anti-T. gondii Th-1-type immune response elicited by pcDNA3-GL vaccination. (A) The levels of anti-T. gondii IgG1 (□) and IgG2a (▪) isotype antibodies in sera of mice injected with two or three doses of pcDNA3 plasmid or pcDNA3-GL 15 weeks after the last immunization. Each experimental group was composed of 10 animals. (B) The lymphoproliferative response of splenocytes from mice immunized with one, two, or three doses of pcDNA3 vector or pcDNA3-GL after stimulation with medium alone (□) or total native T. gondii antigens (▪) 17 weeks after the last immunization. (C) IFN-γ production by T. gondii antigen-stimulated splenocytes from mice immunized with two (□) or three (▪) doses of pcDNA3 vector or pcDNA3-GL 17 weeks after the last injection. Each experimental group was composed of five animals. This study was performed three times with similar results. *, P < 0.05 by the Student's t test (STATSYST; Microsoft). OD 405 nm, optical density at 405 nm. CPM, counts per minute.