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. 2003 Sep;71(9):5436–5439. doi: 10.1128/IAI.71.9.5436-5439.2003

FIG. 1.

FIG. 1.

Analysis of the expression of faeG in transgenic plants. (A) Detection (using RT-PCR) of faeG transcription in transgenic plants. RT-PCR was performed (using specific primers that amplify a 789-bp DNA fragment of faeG) with total RNA from leaves of transgenic tobaccos (32). Lane 1, λDNA (digested with HindIII/EcoRI) molecular weight marker; lanes 2 and 3, RNA from transgenic tobacco; lanes 4 and 5, PCR amplification without RT reaction as a control for DNA contamination; lane 6, RNA from nontransgenic tobacco. (B) Immunoblot detection of recombinant FaeG synthesized in transgenic tobacco. TSP from tobacco leaves was fractionated by sodium dodecyl sulfate-15% polyacrylamide gel electrophoresis. The recombinant FaeG protein was detected with anti-FaeG antibody as the primary antibody and alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody. Lane 1, 0.5 μg of purified recombinant FaeG expressed in E. coli BL21(DE3+K88) as a positive control; lanes 2 and 3, 100 μg each of TSP from transgenic tobacco plants; lane 4, 100 μg of TSP from nontransgenic tobacco.