TABLE 1.

Parameters of rabbit ileal loop ligation analysis^a

Sampleb	Loop length (cm)	Total weight of ileal loop (g)	Weight of empty ileal loop (g)	Vol of accumulated fluid (ml)	V/L
A	6.55 ± 0.07	2.803 ± 0.025	1.206 ± 0.093	1.19 ± 0.01	181.70 ± 4.12
B	6.32 ± 0.47	2.107 ± 0.213	1.107 ± 0.178	0.97 ± 0.12	154.48 ± 15.11
C	6.68 ± 0.58	1.834 ± 0.434	1.349 ± 0.320	0.55 ± 0.29	63.61 ± 22.32
D	6.50 ± 0.28	2.175 ± 0.182	1.850 ± 0.071	0.38 ± 0.11	56.31 ± 15.35

^aThe ratio of accumulated fluid volume to loop length (V/L [in micrograms per centimeter]) was used to express the serum-neutralized effect on K88ad ETEC in ileal loop ligation as previously described (9). A significant difference in neutralizing K88ad ETEC in the sera from the mice immunized with plant-expressed recombinant FaeG was observed in comparison with the sera from the mice immunized with nontransgenic tobacco extracts.

^bA, K88ad ETEC not treated by sera; B, K88ad ETEC treated with the sera of mice immunized with nontransgenic plant leaf extract; C, K88ad ETEC treated with the sera of mice immunized with transgenic plant leaf extract; D, physiological saline solution instead of K88ad ETEC.