TABLE 2.

ORFs and mobile genetic elements within the VAT-PAI

ORF or feature	G + C content (%)	No. of aaa encoded	Positionb (bp)	Gene or protein with similar sequence	% ntc (protein) identity	Accession no.
			1-641	proA (E. coliK-12 MG1655)	95	AE000132
			642-717	thrW (E. coliK-12 MG1655)	100	AE000132
att			699-720	Bacteriophage SflI attachment site (S. flexneri)	100	AF021347
1	39.3	149	1818-2267	Phosphoserine aminotransferase (Spinacea oleracea)	(24)	D84061
2	40.4	60	3736-3918	No significant homology
3	33.8	208	4165-4791	Putative protein (A. thaliana)	(24)	AL049730
4	33.3	89	4801-5070	No significant homology
5	39.1	68	5342-5548	No significant homology
6	36.9	130	5727-6119	No significant homology
7	46.9	117	7362-7715	No significant homology
8	47.1	96	7708-7418	No similarity
9	46.5	164	7732-8226	No significant homology
10	48.2	169	8252-8761	No significant homology
11	47.7	173	8319-7798	No similarity
12	48.8	206	8725-8105	No similarity
13	36.9	65	8926-9123	No significant homology
14	44.5	190	9158-9730	No similarity
15	43.9	84	9654-9400	No similarity
16	44	424	9715-10989	gp22 Mycobacteriophage Bxb1	(30)	AF271693
17	43.6	90	10424-10152	No significant homology
18	41	225	11037-11714	No significant homology
19	46.4	60	11146-10964	No significant homology
20	41.4	61	11164-11349	No significant homology
21	48.3	68	12099-12305	No significant homology
22	44.2	159	12275-11796	Putative integrase (E. coli O157:H7 EDL933)	(33)	AE005658
23	43.2	290	13131-12259	Integrase (X. axonopodis 306)	(27)	AE011739
24	46	112	13389-13727	Integrase (E. coli)	(49)	AJ278144
25	41	130	13831-13439	Integrase (E. coli)	(50)	AJ278144
26	30.5	174	14685-14161	PapX regulatory protein (E. coli)	(48)	P42193
27d	48.4	1377	18944-14811	Tsh and Hbp (E. coli)	80 (75)	AF218073
						AJ223631
28	48.3	71	19534-19749	iso-IS1 insA (S. flexneri 2a)	93 (89)	AF386526.1
29	52.8	64	19831-20025	iso-IS1 insB (S. flexneri 2a)	94 (88)	AF386526.1
30	52.3	64	20012-19818	iso-IS1 insB (S. flexneri 2a)	94 (88)	AF386526.1
31	51.1	74	20206-19982	iso-IS1 insB (S. flexneri 2a)	93 (65)	AF386526.1
att			20421-20571	Bacteriophage SflI attachment site (S. flexneri)	91	AF021347
32	56.7	22	20679-20745	Unknown function (pathogenic poultry E. coli)	86	AF524932.1
33	41.1	63	20709-20900	No similarity
		191	21045-21620	Protein YagU (E. coliK-12 MG1655)	91	AE000136
		76	22138-21908	Ferredoxin (E. coliK-12 MG1655)		AE000136

^aaa, amino acids.

^bData for E. coli K-12 are in bold.

^cnt, nucleotide sequence; percent identity with the translated amino acid sequence is shown in parentheses.

^dORF 27 encodes the Vat protein.