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DNA-binding activities of HSP70 activators in ET-743-treated cells. Extracts were prepared according to the protocol outlined (Top; ref. 26). Nuclear extracts were assayed in the electrophoretic mobility-shift assays with HSE and Y box probes (Middle), and the corresponding CAT activities in the cytoplasmic extracts are shown (Bottom). Even lanes are extracts from heat-shocked cells; uneven lanes are extracts from cells kept at 37°C.
