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. 2003 Sep;71(9):5332–5343. doi: 10.1128/IAI.71.9.5332-5343.2003

FIG. 3.

FIG. 3.

Comparative expression of the ACP-GFP fusion constructs in M. tuberculosis H37Rv (solid bars) and H37Rv:ΔRv3133c (hatched bars). Recombinant bacteria were grown shaking under hypoxic conditions (1.3% O2) supplemented with 5% CO2 for 7 days. Fluorescence readings shown are the means per bacterium (in arbitrary fluorescence units) from the flow-cytometric analysis of recombinant promoter-GFP-expressing M. tuberculosis H37Rv and H37Rv:ΔRv3133c bacteria. Values are the means of two or three independent experiments, and error bars denote standard deviations for each experimental condition. Expression of each of the ACP family promoters was significantly reduced (Student's t test; P < 0.05) in M. tuberculosis H37Rv:ΔRv3133c compared to expression in M. tuberculosis H37Rv.