FIG. 3.

Expression of RTA-responsive genes in KS-1 cells transfected with RTA. Total RNA was extracted from KS-1 cells transfected with pcDNA3 vector alone (Vec) or with pcDNA3/RTA (RTA) at 40 h posttransfection and denatured with formaldehyde. In addition to 5 μg of RNA from transfected KS-1 cells, different amounts of denatured DNA corresponding to the gene of interest were dotted onto nylon membranes and served as the standard (STD). The membranes were subjected to hybridization with gene-specific probes, as described in the legend to FIG. 2.