Anti-CD46 antibodies inhibit binding of species B adenoviruses to CHO-BC1 and A549 cells. The binding of Ad7 (A and C) and Ad11 (B and D) virions to CHO-BC1 (A and B) and A549 (C and D) preincubated with or without (CTRL) antibodies was performed as described in Materials and Methods. GB24 and TRA-2-10, monoclonal antibodies directed against the SCRs 3/4 and 1, respectively; Poly, rabbit anti-human CD46 polyclonal antiserum; RmcB and IF7, monoclonal antibodies directed against human CAR and CD55, respectively; rabbit-1 and rabbit-2, serum from two nonimmunized rabbits.