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. 2003 Sep;77(17):9590–9612. doi: 10.1128/JVI.77.17.9590-9612.2003

FIG. 12.

FIG. 12.

Time course ChIP assays showing the association of C/EBPα, KSHV RTA, and RAP proteins with target viral promoters during early KSHV lytic reactivation. At different time points after TPA induction, aliquots of the BCBL1 cell lysates were precipitated with antibodies against KSHV RTA, RAP, C/EBPα, or CHOP10. Associations between proteins and target promoters were detected by PCR using primers specific for promoter regions of KSHV RTA (A), PAN (B), and MTA (C). Lane 1, DNA size marker; lanes 2 to 5, ChIP assay PCR products from either uninduced input BCBL1 cell lysates (0 h, lanes 6 to 9) or those at 8 h (lanes 10 to 13) and 24 h (lanes 14 to 17) after TPA induction; lanes 6, 10, and 14, PCR products from immunoprecipitates obtained using antibodies against RTA; lanes 7, 11, and 15, PCR products from immunoprecipitates obtained using antibodies against RAP; lanes 8, 12, and 16, PCR products from immunoprecipitates obtained using antibodies against C/EBPα; lanes 9, 13, and 17, PCR products from immunoprecipitates obtained using antibodies against CHOP10; lane 18, size control PCR amplification products from the corresponding promoter reporter plasmids including RTA(−914/+34)-LUC, PAN(−210/+15)-LUC and MTA(−160/+10)-LUC reporter plasmids.