FIG. 5.

Prolongation of survival of HSV-challenged mice by limitin. (A) Twenty-four BALB/c mice were separated into two groups and challenged with HSV (5 × 10⁴ PFU/mouse). The mice were treated with 3 × 10⁴ EU of limitin (solid circle) or PBS (open circles)/mouse on days −1, 0, 1, 2, 3, 4, and 5 relative to HSV exposure. The duration of observation for survival was 14 days. Similar results were obtained in two independent experiments. (B) Eighteen athymic nude mice were separated into two groups and challenged with HSV (10⁴ PFU/mouse). The mice were treated with 3 × 10⁴ EU of limitin (solid circles) or PBS (open circles)/mouse on days −1, 0, 1, 2, 3, 4, and 5 relative to HSV exposure. The duration of observation for survival was 14 days. Similar results were obtained in two independent experiments. Statistical analysis was performed by the Kaplan-Meier method, and the P value was calculated by the generalized Wilcoxon test.