FIG. 4.
Stability of the TβRII protein as determined by pulse-chase analysis in adenovirus-infected cells. (A and D) In two independent experiments, A549 cells were mock infected or infected with rec700 at 50 PFU/cell and maintained in the presence of AraC throughout the infection. At 19 h p.i., cells were pulsed with [35S]methionine-cysteine for 15 min and chased in 10% FCS-supplemented nonradioactive medium for the indicated time periods. Immunoprecipitated, radioactively labeled TβRII was quantified by phosphorimager at each time point of chase (B and E, respectively). (C) Calculations of the TβRII half-life (t1/2).