FIG. 8.

VIRP1-PSTVd RNA in vivo complexes. PSTVd-infected extracts from irradiated tissue were used for IP assays. After IP, the samples were treated with proteinase K, and RNAs were recovered by ethanol precipitation after phenolization. Lane 1, RNAs extracted from IP reaction with a VIRP1Δ-specific polyclonal antibody; lane 2, nonspecific polyclonal antibody used for the IP; lane 3, no antibody was used. Extracted RNAs were separated by PAGE, electroblotted, and hybridized with a α-³²P-labeled negative-strand PSTVd RNA probe.