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Localization of wild-type and mutant MVV Tat-GFP fusion proteins. The TIGEF cell line was transfected with pGFP (A), pGFP-Tat (B), pGFP-5′ (C), and pGFP-3′ (D) plasmid constructs; 48 h posttransfection, the cells were observed by fluorescence microscopy. Magnification, ×400.
