Skip to main content
. 2003 Sep;77(17):9346–9358. doi: 10.1128/JVI.77.17.9346-9358.2003

FIG. 1.

FIG. 1.

ORF K15 expression constructs used in this study and their putative protein products. The K15 ORF is multiply and alternatively spliced (as). The major transcript identified in PEL cells by RT-PCR is fully spliced and contains all eight exons (K15 ex1-8; aa 1 to 489). It encodes a membrane protein with up to 12 transmembrane domains and a cytoplasmic C-terminal domain (aa 355 to 489). The C-terminal domain contains motifs reminiscent of SH2, SH3, and TRAF-like binding sites. The distal TRAF-like binding site and the distal SH2-binding motif Y481EEVL are deleted in construct K15 ex1-8 Δ473 to 489. K15 ex1-8 Y481F carries a point mutation in the distal SH2-binding motif (Y481→F481EEVL). The LMP1-K15355-489 chimera was constructed by fusing the six transmembrane domains of LMP1 to the cytoplasmic C-terminal end (aa 355 to 489) of K15. The splice variants K15 ex1/6-8, K15 ex1 as/6-8, and K15 ex1 as/4-8 differ in the number of transmembrane domains they contain, but all contain the C-terminal domain.